Purification of Hop Stunt Viroid
نویسندگان
چکیده
منابع مشابه
A Viroid Resembling Hop Stunt Viroid in Infected Apple Trees with Apple Scar Skin Disease in the Northeast of Iran
Abstract Background and Aims: Apple scar skin disease (ASSD) is one of the destructive diseases of pome fruits which is caused by ASSVd. Case presentation: We report molecular detection of ASSVd in symptomatic apples cv. Red Delicious with simultaneous presence of a new variant of HSVd from Khorasan Razavi province. Conclusion: This is the first report on the association of HSVd with...
متن کاملComplete nucleotide sequence of a viroid isolated from Etrog citron, a new member of hop stunt viroid group
The c o m p l e t e n u c l e o t i d e s e q u e n c e of a viro i d i s o l a t e d from c i t r u s ( C i t r u s m e d i c a L. U S D C S 6 0 1 3 ) , d e s i g n a t e d as ci t r u s isolate of hop s t u n t v i r o i d ( H S V d t ) ( 1 ) , was e s t a b l i s h e d . T he sin g l e s t r a n d e d c i r c u l a r m o l e c u l e s of c i t r u s v i r o i d RNA c o n s i s t s of 3 02 n ...
متن کاملHop stunt viroid: molecular cloning and nucleotide sequence of the complete cDNA copy.
The complete cDNA of hop stunt viroid (HSV) has been cloned by the method of Okayama and Berg (Mol.Cell.Biol.2,161-170. (1982] and the complete nucleotide sequence has been established. The covalently closed circular single-stranded HSV RNA consists of 297 nucleotides. The secondary structure predicted for HSV contains 67% of its residues base-paired. The native HSV can possess an extended rod-...
متن کاملNucleotide sequence of the Korean strain of hop stunt viroid (HSV).
Hop stunt viroid RNA (HSV) has been detected by molecular hybridization in stunted plants of the Japanese cultivar Kirin II cultivated in Korea (1,2). For propagation, the viroid was transmitted to the wild curcarbU Benincasa Mspida which developed HSV-spedfic severe growth retardation. Isolation, purification, reverse transcription of the viroid-RNA with aid of viroid-specific primers, and seq...
متن کاملNudeotkle Sequence of Cucumber Pale Fruit Virotd: Homology to Hop Stunt Viroid
Double stranded cDNA of cucumber pale fruit viroid (CPFV) has been cloned by the method of Okayama and Berg (Mol.Cell.Biol.2,161-170 (1982)) and the complete nucleotide sequence was established. The covalently closed circular molecules of single-stranded CPFV RNA consists of 303 nucleotides. The nucleotide sequence of CPFV was compared with the previously established sequence of hop stunt viroi...
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ژورنال
عنوان ژورنال: Japanese Journal of Phytopathology
سال: 1982
ISSN: 1882-0484,0031-9473
DOI: 10.3186/jjphytopath.48.182